North, South, or East? Blotting Techniques

East Sensor North Sensor East Motor North Motor South Motor

Robots are generally composed of multiple sensors , actuators and electromechanical parts. The overall behavior of a robot is emergent from coordination among its various parts and its interaction with its environment. Designing a `correct' robot which does`the right thing' in a given environment is an important and challenging problem. The question posed in the title is decomposed into two que...

Vasectomy surgical techniques in South and South East Asia

BACKGROUND Simple ligation of the vas with suture material and excision of a small vas segment is believed to be the most common vasectomy occlusion technique performed in low-resource settings. Ligation and excision (LE) is associated with a risk of occlusion and contraceptive failure which can be reduced by performing fascial interposition (FI) along with LE. Combining FI with intra luminal t...

Molecular biology: Blotting / Hybridization techniques

What is blotting / hybridization? Blotting of nucleic acid is the central technique for hybridization studies. Double stranded DNA will denature or separate at high temperatures into single strands. When the temperature is lowered, the two strands will anneal because of the base pairing interaction between the complementary strands. If nucleotide sequences are identical or similar, complementar...

North–north, North–south, and South–south Relations

Southern Blotting and Related DNA Detection Techniques

Southern blotting is one of the central techniques in molecular biology. First devised by E.M. Southern (1975), Southern blotting results in transfer of DNA molecules, usually restriction fragments, from an electrophoresis gel to a nitrocellulose or nylon sheet (referred to as a ‘membrane’), in such a way that the DNA banding pattern present in the gel is reproduced on the membrane. During tran...